{"id":187545,"date":"2012-01-05T00:00:00","date_gmt":"2012-04-05T14:07:03","guid":{"rendered":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/msr-research-item\/high-throughput-multiscale-modelling-approaches-for-understanding-bacterial-signalling\/"},"modified":"2016-08-23T10:16:18","modified_gmt":"2016-08-23T17:16:18","slug":"high-throughput-multiscale-modelling-approaches-for-understanding-bacterial-signalling","status":"publish","type":"msr-video","link":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/video\/high-throughput-multiscale-modelling-approaches-for-understanding-bacterial-signalling\/","title":{"rendered":"High-throughput, Multiscale modelling approaches for understanding bacterial signalling"},"content":{"rendered":"<div class=\"asset-content\">\n<p>Bacterial chemoreceptor proteins respond to local nutrients and toxins through binding events in receptor domains in the periplasm, causing a conformational change which is propagated into the cell where it triggers a signalling event through downstream effectors and ultimately changes in bacterial motion. Though the biophysical mechanism of signalling through the membrane has been studied extensively through a range of approaches, including mutagenesis of the transmembrane region, the precise mechanism is still unclear. Here I describe a novel high throughput approach to molecular dynamics simulation of transmembrane helices in a bilayer, where the process of building, running and analysing simulations across a cluster is entirely automated. Using this approach, I have been able to identify the role for small (0.15 nm) swinging-piston motions in carrying signals across the membrane. Alongside this, I describe simulations of complete 22 nm chemoreceptor models in a range of realistic environments, from model bilayers to 70 nm vesicles. Taken together, these approaches allow me to propose a mechanism of signal transduction across the membrane.<\/p>\n<\/div>\n<p><!-- .asset-content --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bacterial chemoreceptor proteins respond to local nutrients and toxins through binding events in receptor domains in the periplasm, causing a conformational change which is propagated into the cell where it triggers a signalling event through downstream effectors and ultimately changes in bacterial motion. Though the biophysical mechanism of signalling through the membrane has been studied [&hellip;]<\/p>\n","protected":false},"featured_media":196746,"template":"","meta":{"msr-url-field":"","msr-podcast-episode":"","msrModifiedDate":"","msrModifiedDateEnabled":false,"ep_exclude_from_search":false,"_classifai_error":"","msr_hide_image_in_river":0,"footnotes":""},"research-area":[13553],"msr-video-type":[],"msr-locale":[268875],"msr-post-option":[],"msr-session-type":[],"msr-impact-theme":[],"msr-pillar":[],"msr-episode":[],"msr-research-theme":[],"class_list":["post-187545","msr-video","type-msr-video","status-publish","has-post-thumbnail","hentry","msr-research-area-medical-health-genomics","msr-locale-en_us"],"msr_download_urls":"","msr_external_url":"https:\/\/youtu.be\/J0IyWHRztgc","msr_secondary_video_url":"","msr_video_file":"","_links":{"self":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-video\/187545","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-video"}],"about":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/types\/msr-video"}],"version-history":[{"count":0,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-video\/187545\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/media\/196746"}],"wp:attachment":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/media?parent=187545"}],"wp:term":[{"taxonomy":"msr-research-area","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/research-area?post=187545"},{"taxonomy":"msr-video-type","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-video-type?post=187545"},{"taxonomy":"msr-locale","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-locale?post=187545"},{"taxonomy":"msr-post-option","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-post-option?post=187545"},{"taxonomy":"msr-session-type","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-session-type?post=187545"},{"taxonomy":"msr-impact-theme","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-impact-theme?post=187545"},{"taxonomy":"msr-pillar","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-pillar?post=187545"},{"taxonomy":"msr-episode","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-episode?post=187545"},{"taxonomy":"msr-research-theme","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-theme?post=187545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}