{"id":343280,"date":"2016-12-29T15:46:36","date_gmt":"2016-12-29T23:46:36","guid":{"rendered":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/?post_type=msr-research-item&p=343280"},"modified":"2018-10-16T21:37:59","modified_gmt":"2018-10-17T04:37:59","slug":"nef-mediated-regulation-cd4-hla-class-hiv-1-subtype-c-infection-association-disease-progression-influence-immune-pressure","status":"publish","type":"msr-research-item","link":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/publication\/nef-mediated-regulation-cd4-hla-class-hiv-1-subtype-c-infection-association-disease-progression-influence-immune-pressure\/","title":{"rendered":"Nef-mediated down-regulation of CD4 and HLA class I in HIV-1 subtype C infection: Association with disease progression and influence of immune pressure"},"content":{"rendered":"
\n

Nef plays a major role in HIV-1 pathogenicity. We studied HIV-1 subtype C infected individuals in acute\/early (n<\/em>=120) or chronic (n<\/em>=207) infection to investigate the relationship between Nef-mediated CD4\/HLA-I down-regulation activities and disease progression, and the influence of immune-driven sequence variation on these Nef functions. A single Nef sequence per individual was cloned into an expression plasmid, followed by transfection of a T cell line and measurement of CD4 and HLA-I expression. In early infection, a trend of higher CD4 down-regulation ability correlating with higher viral load set point was observed (r<\/em>=0.19, p<\/em>=0.05), and higher HLA-I down-regulation activity was significantly associated with faster rate of CD4 decline (p<\/em>=0.02). HLA-I down-regulation function correlated inversely with the number HLA-associated polymorphisms previously associated with reversion in the absence of the selecting HLA allele (r<\/em>=\u22120.21, p<\/em>=0.0002). These data support consideration of certain Nef regions in HIV-1 vaccine strategies designed to attenuate the infection course.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Nef plays a major role in HIV-1 pathogenicity. We studied HIV-1 subtype C infected individuals in acute\/early (n=120) or chronic (n=207) infection to investigate the relationship between Nef-mediated CD4\/HLA-I down-regulation activities and disease progression, and the influence of immune-driven sequence variation on these Nef functions. A single Nef sequence per individual was cloned into an […]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"msr-url-field":"","msr-podcast-episode":"","msrModifiedDate":"","msrModifiedDateEnabled":false,"ep_exclude_from_search":false,"_classifai_error":"","msr-author-ordering":null,"msr_publishername":"Elsevier","msr_publisher_other":"","msr_booktitle":"","msr_chapter":"","msr_edition":"Virology","msr_editors":"","msr_how_published":"","msr_isbn":"","msr_issue":"","msr_journal":"Virology","msr_number":"","msr_organization":"","msr_pages_string":"214-225","msr_page_range_start":"214","msr_page_range_end":"225","msr_series":"","msr_volume":"","msr_copyright":"","msr_conference_name":"","msr_doi":"10.1016\/j.virol.2014.08.009","msr_arxiv_id":"","msr_s2_paper_id":"","msr_mag_id":"","msr_pubmed_id":"","msr_other_authors":"","msr_other_contributors":"","msr_speaker":"","msr_award":"","msr_affiliation":"","msr_institution":"","msr_host":"","msr_version":"","msr_duration":"","msr_original_fields_of_study":"","msr_release_tracker_id":"","msr_s2_match_type":"","msr_citation_count_updated":"","msr_published_date":"2014-11-01","msr_highlight_text":"","msr_notes":"","msr_longbiography":"","msr_publicationurl":"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682214003808","msr_external_url":"","msr_secondary_video_url":"","msr_conference_url":"","msr_journal_url":"","msr_s2_pdf_url":"","msr_year":0,"msr_citation_count":0,"msr_influential_citations":0,"msr_reference_count":0,"msr_s2_match_confidence":0,"msr_microsoftintellectualproperty":true,"msr_s2_open_access":false,"msr_s2_author_ids":[],"msr_pub_ids":[],"msr_hide_image_in_river":0,"footnotes":""},"msr-research-highlight":[],"research-area":[13553],"msr-publication-type":[193715],"msr-publisher":[],"msr-focus-area":[],"msr-locale":[268875],"msr-post-option":[],"msr-field-of-study":[],"msr-conference":[],"msr-journal":[],"msr-impact-theme":[],"msr-pillar":[],"class_list":["post-343280","msr-research-item","type-msr-research-item","status-publish","hentry","msr-research-area-medical-health-genomics","msr-locale-en_us"],"msr_publishername":"Elsevier","msr_edition":"Virology","msr_affiliation":"","msr_published_date":"2014-11-01","msr_host":"","msr_duration":"","msr_version":"","msr_speaker":"","msr_other_contributors":"","msr_booktitle":"","msr_pages_string":"214-225","msr_chapter":"","msr_isbn":"","msr_journal":"Virology","msr_volume":"","msr_number":"","msr_editors":"","msr_series":"","msr_issue":"","msr_organization":"","msr_how_published":"","msr_notes":"","msr_highlight_text":"","msr_release_tracker_id":"","msr_original_fields_of_study":"","msr_download_urls":"","msr_external_url":"","msr_secondary_video_url":"","msr_longbiography":"","msr_microsoftintellectualproperty":1,"msr_main_download":"","msr_publicationurl":"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682214003808","msr_doi":"10.1016\/j.virol.2014.08.009","msr_publication_uploader":[{"type":"url","title":"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682214003808","viewUrl":false,"id":false,"label_id":0},{"type":"doi","title":"10.1016\/j.virol.2014.08.009","viewUrl":false,"id":false,"label_id":0}],"msr_related_uploader":"","msr_citation_count":0,"msr_citation_count_updated":"","msr_s2_paper_id":"","msr_influential_citations":0,"msr_reference_count":0,"msr_arxiv_id":"","msr_s2_author_ids":[],"msr_s2_open_access":false,"msr_s2_pdf_url":null,"msr_attachments":[{"id":0,"url":"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682214003808"}],"msr-author-ordering":[{"type":"text","value":"Jaclyn K Mann","user_id":0,"rest_url":false},{"type":"text","value":"Denis Chopera","user_id":0,"rest_url":false},{"type":"text","value":"Saleha Omarjee","user_id":0,"rest_url":false},{"type":"text","value":"Xiaomei T Kuang","user_id":0,"rest_url":false},{"type":"text","value":"Anh Q Le","user_id":0,"rest_url":false},{"type":"text","value":"Gursev Anmole","user_id":0,"rest_url":false},{"type":"text","value":"Ryan Danroth","user_id":0,"rest_url":false},{"type":"text","value":"Philip Mwimanzi","user_id":0,"rest_url":false},{"type":"text","value":"Tarylee Reddy","user_id":0,"rest_url":false},{"type":"user_nicename","value":"carlson","user_id":31333,"rest_url":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/microsoft-research\/v1\/researchers?person=carlson"},{"type":"text","value":"Mopo Radebe","user_id":0,"rest_url":false},{"type":"text","value":"Philip JR Goulder","user_id":0,"rest_url":false},{"type":"text","value":"Bruce D Walker","user_id":0,"rest_url":false},{"type":"text","value":"Salim Abdool Karim","user_id":0,"rest_url":false},{"type":"text","value":"Vladimir Novitsky","user_id":0,"rest_url":false},{"type":"text","value":"Carolyn Williamson","user_id":0,"rest_url":false},{"type":"text","value":"Mark A Brockman","user_id":0,"rest_url":false},{"type":"text","value":"Zabrina L Brumme","user_id":0,"rest_url":false},{"type":"text","value":"Thumbi Ndung'u","user_id":0,"rest_url":false}],"msr_impact_theme":[],"msr_research_lab":[],"msr_event":[],"msr_group":[],"msr_project":[],"publication":[],"video":[],"msr-tool":[],"msr_publication_type":"article","related_content":[],"_links":{"self":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/343280","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item"}],"about":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/types\/msr-research-item"}],"version-history":[{"count":2,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/343280\/revisions"}],"predecessor-version":[{"id":537570,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/343280\/revisions\/537570"}],"wp:attachment":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/media?parent=343280"}],"wp:term":[{"taxonomy":"msr-research-highlight","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-highlight?post=343280"},{"taxonomy":"msr-research-area","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/research-area?post=343280"},{"taxonomy":"msr-publication-type","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-publication-type?post=343280"},{"taxonomy":"msr-publisher","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-publisher?post=343280"},{"taxonomy":"msr-focus-area","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-focus-area?post=343280"},{"taxonomy":"msr-locale","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-locale?post=343280"},{"taxonomy":"msr-post-option","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-post-option?post=343280"},{"taxonomy":"msr-field-of-study","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-field-of-study?post=343280"},{"taxonomy":"msr-conference","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-conference?post=343280"},{"taxonomy":"msr-journal","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-journal?post=343280"},{"taxonomy":"msr-impact-theme","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-impact-theme?post=343280"},{"taxonomy":"msr-pillar","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-pillar?post=343280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}