Bacterium-triggered carious lesions implicate dental hard tissue destruction and the simultaneous initiation of regenerative events comprising dental stem cell activation. Streptococcus mutans (S. mutans) is a prominent pathogen of the oral cavity and the principal cause of caries. S. mutans generates complex products involved in interbacterial interactions, including Mutanobactin-D (Mub-D), which belongs to a group of non-ribosomal cyclic lipopeptides. In the present study, we aimed to analyse the potential role of the synthetic Mub-D peptide in cell populations involved in tissue regenerative processes. To this end, we assessed the in vitro effects of Mub-D in human dental pulp stem cells (hDPSCs) and human bone marrow stem cells (hBMSCs). Our data demonstrated a concentration-dependent effect of Mub-D on their viability and a significant increase in their proliferation and osteogenic\/odontogenic differentiation. These events were associated with specific changes in gene expression, where CCDN-1, RUNX-2, OSX, OCN, DMP-1, DSPP, and BMP-2 genes were upregulated. The ability of Mub-D to modulate the osteogenic\/odontogenic differentiation of both hDPSCs and hBMSCs and considerably enhance mineralisation in a controlled and concentration-dependent manner opens new perspectives for stem cell-based regenerative approaches in the clinics.<\/p>\n","protected":false},"excerpt":{"rendered":"
Bacterium-triggered carious lesions implicate dental hard tissue destruction and the simultaneous initiation of regenerative events comprising dental stem cell activation. Streptococcus mutans (S. mutans) is a prominent pathogen of the oral cavity and the principal cause of caries. S. mutans generates complex products involved in interbacterial interactions, including Mutanobactin-D (Mub-D), which belongs to a group […]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"msr-url-field":"","msr-podcast-episode":"","msrModifiedDate":"","msrModifiedDateEnabled":false,"ep_exclude_from_search":false,"_classifai_error":"","msr-author-ordering":[{"type":"text","value":"Sandra Nikoli\u0107"},{"type":"text","value":"Giuseppe Alastra"},{"type":"user_nicename","value":"Felix Pultar","user_id":"44085"},{"type":"text","value":"Lukas L\u00fcthy"},{"type":"text","value":"B. Stadlinger"},{"type":"text","value":"Erick M. Carreira"},{"type":"text","value":"I. Bugueno"},{"type":"text","value":"T. Mitsiadis"}],"msr_publishername":"","msr_publisher_other":"","msr_booktitle":"","msr_chapter":"","msr_edition":"International Journal of Molecular Sciences","msr_editors":"","msr_how_published":"","msr_isbn":"","msr_issue":"","msr_journal":"International Journal of Molecular Sciences","msr_number":"","msr_organization":"","msr_pages_string":"","msr_page_range_start":"","msr_page_range_end":"","msr_series":"","msr_volume":"","msr_copyright":"","msr_conference_name":"","msr_doi":"10.3390\/ijms26031144","msr_arxiv_id":"","msr_mag_id":"","msr_other_authors":"","msr_other_contributors":"","msr_speaker":"","msr_award":"","msr_affiliation":"","msr_institution":"","msr_host":"","msr_version":"","msr_duration":"","msr_release_tracker_id":"","msr_highlight_type":"","msr_date_display_format":"","msr_main_download_label":"","msr_external_link_label":"","msr_doi_label":"","msr_published_date":"2025-01-28","msr_startdate":"","msr_presentation_date":"","msr_highlight_text":"","msr_notes":"","msr_longbiography":"","msr_publicationurl":"","msr_external_url":"","msr_secondary_video_url":"","msr_conference_url":"","msr_journal_url":"","msr_year":2025,"msr_month":1,"msr_day":28,"msr_microsoftintellectualproperty":false,"msr_pub_id":"d6f41f11ddb7358ebc74f5132b3f1cd9f215d398","msr_publication_uploader":[{"type":"url","title":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/11817755","label_id":243109,"id":false,"viewUrl":false},{"type":"url","title":"https:\/\/pubmed.ncbi.nlm.nih.gov\/39940912","label_id":243109,"id":false,"viewUrl":false},{"type":"url","title":"https:\/\/www.mdpi.com\/1422-0067\/26\/3\/1144\/pdf?version=1738073996","label_id":243132,"id":false,"viewUrl":false}],"msr_related_uploader":[],"msr_original_fields_of_study":[],"msr_s2_paper_id":"","msr_s2_pdf_url":"","msr_citation_count_updated":"","msr_citation_count":0,"msr_influential_citations":0,"msr_reference_count":0,"msr_s2_open_access":false,"msr_s2_author_ids":[],"msr_pub_ids":[{"provider":"s2","id":"d6f41f11ddb7358ebc74f5132b3f1cd9f215d398"},{"provider":"doi","id":"10.3390\/ijms26031144"},{"provider":"pubmed","id":"39940912"},{"provider":"pubmedcentral","id":"11817755"},{"provider":"corpusid","id":"275961038"}],"msr_hide_image_in_river":0,"footnotes":""},"msr-research-highlight":[],"research-area":[],"msr-publication-type":[193715],"msr-publisher":[],"msr-publication-cta":[],"msr-focus-area":[],"msr-locale":[],"msr-post-option":[],"msr-field-of-study":[246985],"msr-conference":[],"msr-journal":[262456],"msr-impact-theme":[],"msr-pillar":[],"class_list":["post-1189894","msr-research-item","type-msr-research-item","status-publish","hentry","msr-field-of-study-medicine"],"msr_publishername":"","msr_edition":"International Journal of Molecular Sciences","msr_affiliation":"","msr_published_date":"2025-01-28","msr_host":"","msr_duration":"","msr_version":"","msr_speaker":"","msr_other_contributors":"","msr_booktitle":"","msr_pages_string":"","msr_chapter":"","msr_isbn":"","msr_journal":"International Journal of Molecular Sciences","msr_volume":"","msr_number":"","msr_editors":"","msr_series":"","msr_issue":"","msr_organization":"","msr_how_published":"","msr_notes":"","msr_highlight_text":"","msr_release_tracker_id":"","msr_original_fields_of_study":"","msr_download_urls":"","msr_external_url":"","msr_secondary_video_url":"","msr_longbiography":"","msr_microsoftintellectualproperty":0,"msr_main_download":"","msr_publicationurl":"","msr_doi":"10.3390\/ijms26031144","msr_publication_uploader":[{"type":"url","title":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/11817755","label_id":243109,"id":false,"viewUrl":false},{"type":"url","title":"https:\/\/pubmed.ncbi.nlm.nih.gov\/39940912","label_id":243109,"id":false,"viewUrl":false},{"type":"url","title":"https:\/\/www.mdpi.com\/1422-0067\/26\/3\/1144\/pdf?version=1738073996","label_id":243132,"id":false,"viewUrl":false}],"msr_related_uploader":[],"msr_citation_count":0,"msr_citation_count_updated":"","msr_s2_paper_id":"","msr_influential_citations":0,"msr_reference_count":0,"msr_arxiv_id":"","msr_s2_author_ids":[],"msr_s2_open_access":false,"msr_s2_pdf_url":null,"msr_attachments":[],"msr-author-ordering":[{"type":"text","value":"Sandra Nikoli\u0107","user_id":0,"rest_url":false},{"type":"text","value":"Giuseppe Alastra","user_id":0,"rest_url":false},{"type":"user_nicename","value":"Felix Pultar","user_id":44085,"rest_url":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/microsoft-research\/v1\/researchers?person=Felix Pultar"},{"type":"text","value":"Lukas L\u00fcthy","user_id":0,"rest_url":false},{"type":"text","value":"B. Stadlinger","user_id":0,"rest_url":false},{"type":"text","value":"Erick M. Carreira","user_id":0,"rest_url":false},{"type":"text","value":"I. Bugueno","user_id":0,"rest_url":false},{"type":"text","value":"T. Mitsiadis","user_id":0,"rest_url":false}],"msr_impact_theme":[],"msr_research_lab":[],"msr_event":[],"msr_group":[],"msr_project":[],"publication":[],"video":[],"msr-tool":[],"msr_publication_type":"article","related_content":[],"_links":{"self":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/1189894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item"}],"about":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/types\/msr-research-item"}],"version-history":[{"count":1,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/1189894\/revisions"}],"predecessor-version":[{"id":1189895,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-item\/1189894\/revisions\/1189895"}],"wp:attachment":[{"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/media?parent=1189894"}],"wp:term":[{"taxonomy":"msr-research-highlight","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-research-highlight?post=1189894"},{"taxonomy":"msr-research-area","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/research-area?post=1189894"},{"taxonomy":"msr-publication-type","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-publication-type?post=1189894"},{"taxonomy":"msr-publisher","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-publisher?post=1189894"},{"taxonomy":"msr-publication-cta","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-publication-cta?post=1189894"},{"taxonomy":"msr-focus-area","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-focus-area?post=1189894"},{"taxonomy":"msr-locale","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-locale?post=1189894"},{"taxonomy":"msr-post-option","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-post-option?post=1189894"},{"taxonomy":"msr-field-of-study","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-field-of-study?post=1189894"},{"taxonomy":"msr-conference","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-conference?post=1189894"},{"taxonomy":"msr-journal","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-journal?post=1189894"},{"taxonomy":"msr-impact-theme","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-impact-theme?post=1189894"},{"taxonomy":"msr-pillar","embeddable":true,"href":"https:\/\/cm-edgetun.pages.dev\/en-us\/research\/wp-json\/wp\/v2\/msr-pillar?post=1189894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}